Sp Column Free From Impurity

Types of columns: 1- Gravity Columns: The mobile phase move through the stationary phase by gravity force. 2- Flash Columns (Air.

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There are a number of different kinds of chromatography, which differ in the mobile and the stationary phase used.

This is a solid - liquid technique in which the stationary phase is a solid mobile phase is a liquid.

PRINCIPLE Adsorption Mixture of components dissolved in the M.P is introduced in to the column.

In contrast, a compound more strongly attracted to the stationary phase will move slowly through the column.

Silica gel gives good results with compounds containing polar functional groups.

The solvents should also have sufficiently low boiling points to permit ready recovery of eluted material.

However, polarity as seen the most important factor in adsorption chromatography.

Different mobile phases used: ( in increasing order of polarity) Petroleum ether, carbon tetrachloride, cyclohexane, ether, acetone, benzene, toluene, esters, water, etc It can b e used in either pure form or as mixture of solvents.

The material of the column is mostly good quality neutral glass since it shouldnt be affected by solvents.

An ordinary burette can also be used as column for separation.

Column dimensions - length diameter ratio (10:1,30:1 or 100:1) Various accessories are attached to the top and bottom of the column for maintenance of the elution process.

Sp Column Free From Impurity

Wet packing wet filling The column should be free from impurity, before using column, it should be washed properly and dry it.

Before filling column with stationary phase, cottonglass wool is kept It should be uniformly filled.

The entire sample is introduced into the column at once and get adsorbed on the top portion of the column.

From this zone, individual sample can be separated by a process of elution.

The two techniques are: (i) Isocratic elution technique: in this elution technique, same solvent composition or solvent of same polarity is used throughout the process of separation.

If the compounds to be isolated from column chromatography are colorless.

In this case, small fractions of the eluent are collected sequentially in labelled tubes and the composition of each fraction is analyzed by TLC.

Fractions can be collected in test tubes, vials, beakers, or Erlenmeyer flasks.

Particle size of column packing: separation to be improved by decreasing the particle size of the adsorbent.

Activity of the adsorbent Temperature of the column: The speed of the elution increases at higher temperatures.

Packing of the column Quality of solvents: solvents having low viscosities is giving better results.

Types of columns: 1- Gravity Columns: The mobile phase move through the stationary phase by gravity force.

Flash Columns (Air.

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